 ที่มา:Geodermaหัวเรื่อง:Surface charge properties of kaolinite from Thai soils |
 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Hybridity Test of Cucumbers via Ultrathin Layer Isoelectric Focusing Technique Using Water as the Extraction Buffer) ผู้เขียน: Damrongvudhi Onwimol,  ดร.เสริมศิริ จันทร์เปรม, ผู้ช่วยศาสตราจารย์ , ดร.ธรรมศักดิ์ ทองเกตุ, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe ultrathin layer isoelectric focusing (UTLIEF) technique using water as the extraction buffer is a cheap, fast and reliable technique for varietal identification in cucumber. However, its application for hybridity testing has not been reported yet. Therefore, this study evaluated the possibility of the application of the UTLIEF technique for testing the hybridity of 10 F1 hybrid seeds and their respective parental lines. It was found that nine out of the ten pairs of the parental lines could be differentiated by the differences in the combination of male and female marker bands, while eight out of the ten F1 hybrid seed were verified using only male marker bands using this UTLIEF technique with water as the protein extract solvent and a gel pH range of 2–11. More male marker bands were found than female marker bands. Most of the polymorphic cucumber seed protein bands were focused in the pH range 6–10. The results indicated that the UTLIEF technique using water as the extraction buffer and a gel pH gradient of 2–11 is of value for hybridity testing in commercial cucumber hybrid seed production. |
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ผลงานตีพิมพ์ในวารสารวิชาการCharacterization of doped TiO2 nanoparticle dispersionsผู้แต่ง:Sahu, M., Suttiponparnit, K., Dr.Sirikalaya Suvachittanont, Associate Professor , Charinpanitkul, T., Biswas, P., วารสาร:  
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Seed Storage Protein Extraction and Gel pH gradient for Cucumber Varietal Identification via an Ultrathin-Layer Isoelectric Focusing Technique) ผู้เขียน:ดำรงวุฒิ อ่อนวิมล, Sermsiri Chanpreme, ดร.ธรรมศักดิ์ ทองเกตุ, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractA suitable seed protein extract solution and gel pH gradient for cucumber varietal identification via an ultrathin-layer isoelectric focusing (UTLIEF) technique were studied. Four seed protein extract solutions, namely; water, phosphate buffer, Na2EDTA and NaCl in combination with 2 gel pH gradients of 2-11 and 4-5/3-10 were used with UTLIEF carried out on four commercial F-1 hybrid cucumber cultivars namely; Micro C, Big C, Chok-Dee and Bussaba. The results showed that the phosphate buffer solution dissolved most cucumber seed protein, resulting in the highest total protein concentration, followed by water solvent. Most polymorphic cucumber seed protein bands were focused in the pH range of 7-10. The pH gradient of 2-11 showed sharper and clearer protein bands than that of pH gradient 4-5/3-10. Water-extracted seed protein run on in a gel pH gradient of 2-11 had the most polymorphic protein band markers that could completely differentiate the four cucumber cultivars from each other. |
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ที่มา:Agricultural Science Journal (วารสารวิทยาศาสตร์เกษตร)หัวเรื่อง:การสกัดโปรตีนที่สะสมในเมล็ดเพื่อนำมาใช้แยกความแตกต่างของสายพันธุ์ข้าวโพดหวานด้วยเทคนิค Ultrathin-layer Isoelectric Focusing |
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